Spectroscopy - June 2009 - (Page 41) w w w. s p e c t r o s c o p y o n l i n e . c o m June 2009 Spectroscopy 24(6) 41 appropriate DNA. This clearly suggests that [Ru(bpy)2(BTIP)]2+ interacts with DNA most likely through a stacking interaction between the aromatic chromophore and the DNA base pairs. Hence, [Ru(bpy)2(BTIP)]2+ clearly binds to DNA by intercalation. The corresponding relationships of the RLS spectra and the absorption spectra show clearly. The RLS peaks of 315 and 374 nm correspond to the absorbance valley, and the RLS valley at about 452 nm corresponds to the peak of absorbance. Furthermore, the RLS intensity increases in the presence of DNA while the absorbance decreases. This could be elucidated with the theory of resonance light scattering (5,6). Optimization of Conditions pH effect Figure 3 shows the relationship between the RLS intensity and solution pH of the system. The effect of pH on the RLS is investigated from pH 2.0 to 10.0. Figure 4 exhibits that the enhancement of light-scattering changes a great deal and reaches a maximum with pH 6.0. [Ru(bpy)2(BTIP)]2+ has low RLS signals in this range, whereas DNA has a high RLS intensity and the pH is lower than 3.5, which might be due to DNA aggregate forming large particles whose dimensions are comparable to the wavelength of UV–vis light and result in strong light scattering (41). But DNA has low RLS signals when the pH is higher than 3.5, so pH 6.0 is chosen for further research. Effect of the [Ru(bpy)2(BTIP)]2+ concentration The effect of [Ru(bpy)2(BTIP)]2+ concentration on IRLS of the [Ru(bpy)2(BTIP)]2+ctDNA system is investigated and shown in Figure 4. The [Ru(bpy)2(BTIP)]2+ concentration has an obvious effect on I RLS of the [Ru(bpy)2 (BTIP)]2+ ctDNA system. The I RLS increases with the increasing concentration of [Ru(bpy)2(BTIP)]2+. Yet when the concentration of [Ru(bpy)2 (BTIP)]2+ is higher than 2.0 × 10 6 mol/L, the IRL2+ Sof the [Ru(bpy)2(BTIP)] -ctDNA system starts to decrease, which could be caused by the high absorbance of large concentrations of [Ru(bpy)2(BTIP)]2+. So 2.0 × 10 6 mol/L, which caused the maximum IRLS, is selected for the following procedure. Influence of the addition order of the reagent and the stability Three kinds of reagent addition orders are investigated, and all the measurements are done after the solutions have Table I: The effect of adding orders ctDNA: 1.08 mg/L; [Ru(bpy)2(BTIP)]2+: 2.0 × 10 Number 1 2 3 6 mol/L; BR: pH = 6.0 I RLS(a.u.) 388.99 327.80 289.98 Addition order of reagents [Ru(bpy)2(BTIP)]2+ + Buffer + ctDNA Buffer + ctDNA + [Ru(bpy)2 (BTIP)]2+ [Ru(bpy)2(BTIP)]2++ ctDNA + Buffer Figure 3: Effect of pH on the IRLS value of the [Ru(bpy)2(BTIP)]2+-ctDNA system. Conditions: (BR) pH = 6; (a) [Ru(bpy)2(BTIP)]2+: 2.0 × 10 -6 mol/L; (b) ctDNA: 1.08 mg/L; and(c) [Ru(bpy)2(BTIP)]2++ ctDNA. Concentration (10 -6moIL-1) Figure 4: Effect of different concentrations of Ru(bpy)2(BTIP) 2+on the RLS signals in Ru(bpy)2(BTIP) 2+ctDNA system. ctDNA:1.08 mg/L; BR pH 6.0. B: [Ru(bpy)2(BTIP)]2+; D: [Ru(bpy)2(BTIP)]2++ ctDNA. http://www.spectroscopyonline.com Table of Contents for the Digital Edition of Spectroscopy - June 2009 Spectroscopy - June 2009 Contents News Spectrum Auger Spectroscopy Statistics and Chemometrics for Clinical Data Reporting, Part 1 Understanding and Interpreting the New GAMP 5 Software Categories Current Status of Standoff LIBS Security Applications at the United States Army Research Laboratory Spectral Studies on the Interaction of [Ru(bpy)2(BTIP)]2+ with DNA and Determination of Nucleic Acids at Nanogram Levels Product Resources Calendar Ad Index Spectroscopy - June 2009 Spectroscopy - June 2009 - (Page BB1) Spectroscopy - June 2009 - (Page BB2) Spectroscopy - June 2009 - Spectroscopy - June 2009 (Page Cover1) Spectroscopy - June 2009 - Spectroscopy - June 2009 (Page Cover2) Spectroscopy - June 2009 - Spectroscopy - June 2009 (Page 3) Spectroscopy - June 2009 - Spectroscopy - June 2009 (Page 4) Spectroscopy - June 2009 - Spectroscopy - June 2009 (Page 5) Spectroscopy - June 2009 - Spectroscopy - June 2009 (Page 6) Spectroscopy - June 2009 - Spectroscopy - June 2009 (Page 7) Spectroscopy - June 2009 - Contents (Page 8) Spectroscopy - June 2009 - Contents (Page 9) Spectroscopy - June 2009 - Contents (Page 10) Spectroscopy - June 2009 - Contents (Page 11) Spectroscopy - June 2009 - News Spectrum (Page 12) Spectroscopy - June 2009 - News Spectrum (Page 13) Spectroscopy - June 2009 - Auger Spectroscopy (Page 14) Spectroscopy - June 2009 - Auger Spectroscopy (Page 15) Spectroscopy - June 2009 - Auger Spectroscopy (Page 16) Spectroscopy - June 2009 - Auger Spectroscopy (Page 17) Spectroscopy - June 2009 - Statistics and Chemometrics for Clinical Data Reporting, Part 1 (Page 18) Spectroscopy - June 2009 - Statistics and Chemometrics for Clinical Data Reporting, Part 1 (Page 19) Spectroscopy - June 2009 - Statistics and Chemometrics for Clinical Data Reporting, Part 1 (Page 20) Spectroscopy - June 2009 - Statistics and Chemometrics for Clinical Data Reporting, Part 1 (Page 21) Spectroscopy - June 2009 - Understanding and Interpreting the New GAMP 5 Software Categories (Page 22) Spectroscopy - June 2009 - Understanding and Interpreting the New GAMP 5 Software Categories (Page 23) Spectroscopy - June 2009 - Understanding and Interpreting the New GAMP 5 Software Categories (Page 24) Spectroscopy - June 2009 - Understanding and Interpreting the New GAMP 5 Software Categories (Page 25) Spectroscopy - June 2009 - Understanding and Interpreting the New GAMP 5 Software Categories (Page 26) Spectroscopy - June 2009 - Understanding and Interpreting the New GAMP 5 Software Categories (Page 27) Spectroscopy - June 2009 - Understanding and Interpreting the New GAMP 5 Software Categories (Page 28) Spectroscopy - June 2009 - Understanding and Interpreting the New GAMP 5 Software Categories (Page 29) Spectroscopy - June 2009 - Understanding and Interpreting the New GAMP 5 Software Categories (Page 30) Spectroscopy - June 2009 - Understanding and Interpreting the New GAMP 5 Software Categories (Page 31) Spectroscopy - June 2009 - Current Status of Standoff LIBS Security Applications at the United States Army Research Laboratory (Page 32) Spectroscopy - June 2009 - Current Status of Standoff LIBS Security Applications at the United States Army Research Laboratory (Page 33) Spectroscopy - June 2009 - Current Status of Standoff LIBS Security Applications at the United States Army Research Laboratory (Page 34) Spectroscopy - June 2009 - Current Status of Standoff LIBS Security Applications at the United States Army Research Laboratory (Page 35) Spectroscopy - June 2009 - Current Status of Standoff LIBS Security Applications at the United States Army Research Laboratory (Page 36) Spectroscopy - June 2009 - Current Status of Standoff LIBS Security Applications at the United States Army Research Laboratory (Page 37) Spectroscopy - June 2009 - Current Status of Standoff LIBS Security Applications at the United States Army Research Laboratory (Page 38) Spectroscopy - June 2009 - Spectral Studies on the Interaction of [Ru(bpy)2(BTIP)]2+ with DNA and Determination of Nucleic Acids at Nanogram Levels (Page 39) Spectroscopy - June 2009 - Spectral Studies on the Interaction of [Ru(bpy)2(BTIP)]2+ with DNA and Determination of Nucleic Acids at Nanogram Levels (Page 40) Spectroscopy - June 2009 - Spectral Studies on the Interaction of [Ru(bpy)2(BTIP)]2+ with DNA and Determination of Nucleic Acids at Nanogram Levels (Page 41) Spectroscopy - June 2009 - Spectral Studies on the Interaction of [Ru(bpy)2(BTIP)]2+ with DNA and Determination of Nucleic Acids at Nanogram Levels (Page 42) Spectroscopy - June 2009 - Spectral Studies on the Interaction of [Ru(bpy)2(BTIP)]2+ with DNA and Determination of Nucleic Acids at Nanogram Levels (Page 43) Spectroscopy - June 2009 - Spectral Studies on the Interaction of [Ru(bpy)2(BTIP)]2+ with DNA and Determination of Nucleic Acids at Nanogram Levels (Page 44) Spectroscopy - June 2009 - Product Resources (Page 45) Spectroscopy - June 2009 - Product Resources (Page 46) Spectroscopy - June 2009 - Product Resources (Page 47) Spectroscopy - June 2009 - Calendar (Page 48) Spectroscopy - June 2009 - Calendar (Page 49) Spectroscopy - June 2009 - Ad Index (Page 50) Spectroscopy - June 2009 - Ad Index (Page Cover3) Spectroscopy - June 2009 - Ad Index (Page Cover4) http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0810_bg http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0810_supp http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0710 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0610 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0510 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0410 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0310 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0210 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0110 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy1209 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy1109 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy1009 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0909 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0809 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0709 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0609 http://digital.findanalytichem.com/nxtbooks/advanstar/spectroscopy0509 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0409 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0309 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0209 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0109 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy1208 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy1108 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy1008 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0908 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0808 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0708 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0608 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0508 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0308 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0208 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0108 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy1207 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy1107 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy1007 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0907 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0807 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0707 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0607 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0507 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0407 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0307 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0207 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0107 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy1106 http://www.nxtbook.com/nxtbooks/advanstar/spectroscopy0906 http://www.nxtbookMEDIA.com
For optimal viewing of this digital publication, please enable JavaScript and then refresh the page. If you would like to try to load the digital publication without using Flash Player detection, please click here.